Limouse C, Smith OK, Jukam D, Fryer KA, Greenleaf WJ, Straight AF. Global mapping of RNA-chromatin contacts reveals a proximity-dominated connectivity model for ncRNA-gene interactions. Nat Commun. 2023 Sep 28;14(1):6073. doi: 10.1038/s41467-023-41848-9. PMID: 37770513; PMCID: PMC10539311.

Sidhwani P, Straight AF. Epigenetic inheritance and boundary maintenance at human centromeres. Curr Opin Struct Biol. 2023 Oct;82:102694. doi: 10.1016/ Epub 2023 Aug 30. PMID: 37657353; PMCID: PMC10530090.

Flores Servin JC, Brown RR, Straight AF. Repression of CENP-A assembly in metaphase requires HJURP phosphorylation and inhibition by M18BP1. J Cell Biol. 2023 Jun 5;222(6):e202110124. doi: 10.1083/jcb.202110124. Epub 2023 May 4. PMID: 37141119; PMCID: PMC10165474.

Sundararajan K, Straight AF. Centromere Identity and the Regulation of Chromosome Segregation. Front Cell Dev Biol. 2022 Jun 2;10:914249. doi: 10.3389/fcell.2022.914249. PMID: 35721504; PMCID: PMC9203049.

Zhou K, Gebala M, Woods D, Sundararajan K, Edwards G, Krzizike D, Wereszczynski J, Straight AF*, Luger K*. CENP-N promotes the compaction of centromeric chromatin. Nature Structural & Molecular Biology. 2022 *correspondence.

Altemose N1, Maslan A1, Smith OK1, Sundararajan K1, Brown RR, Mishra R, Detweiler AM, Neff N, Miga KH, Straight AF*, Streets A*. DiMeLo-seq: a long-read, single-molecule method for mapping protein–DNA interactions genome wide. Nature Methods. 2022 1co-first author, *correspondence.

Hoyt SJ, Storer JM#, Hartley GA#, Grady PGS#, Gershman A#, de Lima LG, Limouse C, Halabian R, Wojenski L, Rodriguez M, Altemose N, Rhie A, Core LJ, Gerton JL, Makalowski W, Olson D, Rosen J, Smit AFA, Straight AF, Vollger MR, Wheeler TJ, Schatz MC, Eichler EE, Phillippy AM, Timp W, Miga KH, O’Neill RJ. From telomere to telomere: The transcriptional and epigenetic state of human repeat elements. Science. 2022 376(6588):eabk3112. #equal contribution.

Altemose N, Logsdon GA#, Bzikadze AV#, Sidhwani P#, Langley SA#, Caldas GV#, Hoyt SJ, Uralsky L, Ryabov FD, Shew CJ, Sauria MEG, Borchers M, Gershman A, Mikheenko A, Shepelev VA, Dvorkina T, Kunyavskaya O, Vollger MR, Rhie A, McCartney AM, Asri M, Lorig-Roach R, Shafin K, Lucas JK, Aganezov S, Olson D, de Lima LG, Potapova T, Hartley GA, Haukness M, Kerpedjiev P, Gusev F, Tigyi K, Brooks S, Young A, Nurk S, Koren S, Salama SR, Paten B, Rogaev EI, Streets A, Karpen GH, Dernburg AF, Sullivan BA, Straight AF, Wheeler TJ, Gerton JL, Eichler EE, Phillippy AM, Timp W, Dennis MY, O’Neill RJ, Zook JM, Schatz MC, Pevzner PA, Diekhans M, Langley CH, Alexandrov IA, Miga KH. Complete genomic and epigenetic maps of human centromeres. Science. 2022 376(6588):eabl4178. #equal contribution.

Jukam D, Kapoor RR, Straight AF*, Skotheim JM*. The DNA-to-cytoplasm ratio broadly activates zygotic gene expression in Xenopus. Current Biology. 2021 31:1-13. *correspondence.

Altemose N1, Maslan A1, Smith OK1, Sundararajan K1, Brown RR, Detweiler AM, Neff N, Miga KH, Straight AF*, Streets A*. DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome-wide. bioRxiv. 2021 doi: 1co-first author, *correspondence.

Smith OK, Limouse C, Fryer KA, Teran NA, Sundararajan K, Heald R, Straight AF. Identification and characterization of centromeric sequences in Xenopus laevis. Genome Research. 2021; Jun;31(6):958-967. doi: 10.1101/gr.267781.120. PMID: 33875480

Limouse C, Jukam D, Smith OK, Fryer KA, Straight AF. Mapping Transcriptome-Wide and Genome-Wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq). Methods Mol Biol. 2020;2161:115-142. doi: 10.1007/978-1-0716-0680-3_10. PMID: 32681510

Jukam D, Limouse C, Smith OK, Risca VI, Bell JC, Straight AF. Chromatin-Associated RNA Sequencing (ChAR-seq). Curr Protoc Mol Biol. 2019 Apr;126(1):e87. doi: 10.1002/cpmb.87. Epub 2019 Feb 20. PMID: 30786161

French BT, Straight AF. CDK phosphorylation of Xenopus laevis M18BP1 promotes its metaphase centromere localization. EMBO J. 2019 Jan 2. pii: e100093. doi: 10.15252/embj.2018100093. PMID: 30606714

Bell JC, Jukam D, Teran NA, Risca VI, Smith OK, Johnson WL, Skotheim JM, Greenleaf WJ, Straight AF. Chromatin-associated RNA sequencing (ChAR-seq) maps genome-wide RNA-to-DNA contacts. Elife. 2018 Apr 12;7. pii: e27024. doi: 10.7554/eLife.27024. PMID: 29648534 

Limouse C, Bell JC, Fuller CJ, Straight AF, Mabuchi H. Measurement of Mesoscale Conformational Dynamics of Freely Diffusing Molecules with Tracking FCS. Biophys J. 2018 Apr 10;114(7):1539-1550. doi: 10.1016/j.bpj.2018.01.044. PMID: 29642025

Flores Servin JC, Straight AF. Centromere and Kinetochore Assembly in Xenopus laevis Egg Extract. Cold Spring Harb Protoc. 2018 Sep 4;2018(9):pdb.prot102509. doi: 10.1101/pdb.prot102509. PMID: 29475995

Cao S, Zhou K, Zhang Z, Luger K, Straight AF. Constitutive centromere-associated network contacts confer differential stability on CENP-A nucleosomes in vitro and in the cell. Mol Biol Cell. 2018 Mar 15;29(6):751-762. doi: 10.1091/mbc.E17-10-0596. Epub 2018 Jan 17.
PMID: 29343552 

French BT, Straight AF. The Power of Xenopus Egg Extract for Reconstitution of Centromere and Kinetochore Function. Prog Mol Subcell Biol. 2017 Aug 25; 56:59-84. doi: 10.1007/978-9-319-58592-5_3. PubMed PMID: 28840233.

Johnson WL, Yewdell WT, Bell JC, McNulty SM, Duda Z, O’Neill RJ, Sullivan BA, Straight AF. RNA-dependent stabilization of SUV39H1 at constitutive heterochromatin.  Elife. 2017 Aug 1;6. Pii: e25299. doi: 10.7554/eLife.25299. PubMed PMID: 2760200.

French BT, Westhrope FG, Limouse C, Straight AF. Xenopus laveis M18BP1 Directly Binds Existing CENP-A Nucleosomes to Promote Centromeric Chromatin Assembly. Dev Cell. 2017 Jul 24; 42(2):190-199.e10. doi: 10.1016/j.devcel.2017.06.021. PubMed PMID: 28743005.

Foisner R, Straight AF. Editorial overview: The cell nucleus: New discoveries on nuclear structure, dynamics and function.  Curr Opin Cell Biol. 2017 Jun;46:iv-vi. doi: 10.1016/ PubMed PMID: 28693757.

Johnson WL, Straight AF. RNA-mediated regulation of heterochromatinCurr Opin cell Biol. 2017 Jun 11;46:102-109. doi:10.1016/ PubMed PMID: 28614747.

Eser U, Chandler-Brown D, Ay F, Straight AF, Duan Z, Nobel WS, Skotheim JM.  Form and function of topologically associating genomic domains in budding yeast. Proc Natl Acad Sci USA. 2017 Apr 11;114(15):E3061-E3070. doi: 10.1073/pnas. 1612256114. PubMed PMID: 28348222.

Risca VI, Denny SK, Straight AF, Greenleaf WJ. Variable chromatin structure revealed by in situ spatially correlated DNA cleavage mapping. Nature. 2017 Jan 12;54541(7636):237-241. doi: 10.1038/nature20781. PubMed PMID: 28024297.  

Westhorpe FG, Straight AF.  Chromosomes Segregation: Reconstituting the KinetochoreCurr Biol. 2016 Dec 5;26(23):R1242-R1245. doi:10.1016/j.cub.2016.09.051. PubMed PMID: 27923136. 

Ladurner R, Straight AF. MIS12/MIND Control at the Kinetochore.  Cell. 2016 Nov3;167(4):889-891. doi: 10.1016/j.cell.2016.10.036. PubMed PMID: 27814517.

Shang WH, Hori T, Westhorpe FG, Godek KM, Toyoda A, Misu S, Monma N, Ikeo K, Carroll CW, Takami Y, Fujiyama A, Kimura H, Straight AF, Fukagawa T.  Acetylation of histone H4 lysine 5 and 12 is required for CENP-A deposition into centromeres.  Nat Commun. 2016 Nov 4;7:13465. doi: 10.1038/ncomms13465. PubMed PMID: 27811920.

Bell JC, Straight AF. Condensing chromosome condensation. Nat Cell Biol. 2015 Jul 31;17(8):964-5. doi: 10.1038/ncb3212. PubMed PMID: 26239527.

Westhorpe FG, Fuller CJ, Straight AF. A cell-free CENP-A assembly system defines the chromatin requirements for centromere maintenance. J Cell Biol. 2015 Jun 22;209(6):789-801. doi: 10.1083/jcb.201503132. PubMed PMID: 26076692.

Amodeo AA, Jukam D, Straight AF*, Skotheim JM*. Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):E1086-95. doi: 10.1073/pnas.1413990112. PubMed PMID: 25713373. *Corresponding Author

Miell MD, Straight AF. Regulating the timing of CENP-A nucleosome assembly by phosphorylation. Dev Cell. 2015 Jan 12;32(1):1-2. doi: 10.1016/j.devcel.2014.12.020. PubMed PMID: 25584791.

Westhorpe FG, Straight AF. The centromere: epigenetic control of chromosome segregation during mitosis. Cold Spring Harb Perspect Biol. 2014 Nov 20;7(1):a015818. doi: 10.1101/cshperspect.a015818. PubMed PMID: 25414369.

French BT, Straight AF. Swapping CENP-A at the centromere. Nat Cell Biol. 2013Sep;15(9):1028-30. doi: 10.1038/ncb2833. PubMed PMID: 23999616.

Johnson WL, Straight AF. Fluorescent protein applications in microscopy.Methods Cell Biol. 2013;114:99-123. doi: 10.1016/B978-0-12-407761-4.00005-1.PubMed PMID: 23931504.

Kato H, Jiang J, Zhou BR, Rozendaal M, Feng H, Ghirlando R, Xiao TS, Straight AF, Bai Y. A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C. Science. 2013 May 31;340(6136):1110-3. doi:10.1126/science.1235532. PubMed PMID: 23723239; PubMed Central PMCID: PMC3763809.

Miell MD, Fuller CJ, Guse A, Barysz HM, Downes A, Owen-Hughes T, Rappsilber J,Straight AF, Allshire RC. CENP-A confers a reduction in height on octamericnucleosomes. Nat Struct Mol Biol. 2013 Jun;20(6):763-5. doi: 10.1038/nsmb.2574.Epub 2013 May 5. PubMed PMID: 23644598; PubMed Central PMCID: PMC3676451.

Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, CheesemanIM, Choo KH, Copenhaver GP, Deluca JG, Desai A, Diekmann S, Erhardt S,Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM,Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LE, Karpen G, Kops GJ, LampsonMA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RL, Masumoto H,McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O’Neill RJ, Salmon ED,Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE,Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L,Yanagida M, Yen TJ, Yoda K, Cleveland DW. Esperanto for histones: CENP-A, notCenH3, is the centromeric histone H3 variant. Chromosome Res. 2013Apr;21(2):101-6. doi: 10.1007/s10577-013-9347-y. Epub 2013 Apr 12. PubMed PMID:23580138; PubMed Central PMCID: PMC3627038. 

Westhorpe FG, Straight AF. Functions of the centromere and kinetochore inchromosome segregation. Curr Opin Cell Biol. 2013 Jun;25(3):334-40. doi:10.1016/ Epub 2013 Mar 13. Review. PubMed PMID: 23490282;PubMed Central PMCID: PMC3687001.

Guse A, Fuller CJ, Straight AF. A cell-free system for functional centromereand kinetochore assembly. Nat Protoc. 2012 Oct;7(10):1847-69. doi:10.1038/nprot.2012.112. Epub 2012 Sep 20. PubMed PMID: 23018190; PubMed CentralPMCID: PMC4011387.

Fuller CJ, Straight AF. Imaging nanometre-scale structure in cells using insitu aberration correction. J Microsc. 2012 Oct;248(1):90-101. doi:10.1111/j.1365-2818.2012.03654.x. Epub 2012 Aug 20. PubMed PMID: 22906048; PubMedCentral PMCID: PMC3773235.

Westhorpe FG, Straight AF. The split personality of CENP-A nucleosomes. Cell. 2012 Jul 20;150(2):245-7. doi: 10.1016/j.cell.2012.07.003. PubMed PMID: 22817887;PubMed Central PMCID: PMC3724509.

Hellwig, D., Emmerth, S., Ulbricht, T., Döring, V, Hoischen, C., Martin, R., Samora, C.P., McAinsh, A.D., Carroll, C.W.,Straight, A.F., Meraldi, P., Diekmann, S. (2011) Dynamics of CENP-N kinetochore binding during the cell cycle.J Cell Sci. 124 (Pt 22): 3871-83.

Guse, A., Carroll, C.W., Moree, B., Fuller, C.J. and Straight, A.F. (2011) In vitro centromere and kinetochore assembly on defined chromatin templates.Nature. 477 (7364): 354-8.

Moree B, Meyer CB, Fuller CJ, Straight AF. CENP-C recruits M18BP1 tocentromeres to promote CENP-A chromatin assembly. J Cell Biol. 2011 Sep19;194(6):855-71. doi: 10.1083/jcb.201106079. Epub 2011 Sep 12. PubMed PMID:21911481; PubMed Central PMCID: PMC3207292.

Koslover EF, Fuller CJ, Straight AF, Spakowitz AJ. (2010) Local geometry and elasticity in compact chromatin structure.Biophysical Journal. 99 (12): 3941-50.

Carroll CW, Milks KJ, Straight AF. (2010) Dual recognition of CENP-A nucleosomes is required for centromere assembly.J Cell Biol. 189 (7): 1143-55.

Sage, J and Straight AF. (2010) RB’s original CIN? Genes Dev. 24 (13): 1329-33.

Fuller CJ and Straight AF. (2009) Image Analysis Benchmarking Methods for High Content Screen Design. J. Microscopy. 17 (17): 145-161.

Milks KJ; Moree B; Straight AF. (2009) Dissection of CENP-C-directed Centromere and Kinetochore Assembly. Mol Biol Cell. 20 (19): 4246-55.

Carroll CW; Silva MC; Godek KM; Jansen LE; Straight AF. (2009) Centromere assembly requires the direct recognitionof CENP-A nucleosomes by CENP-N. Nat Cell Biol. 11 (7): 896-902.

Erhardt S. Mellone BG, Betts CM, Zhang W, Karpen GH, Straight AF. (2008)Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation.J Cell Biol. 1; 183(5): 805-18.

Brennan IM, Peters U, Kapoor TM, Straight AF. (2007) Polo-like kinase controls vertebrate spindle elongation and cytokinesis. PLoS ONE. 2 (5): e409.